Animal Science Faculty

Kevin Wells

Kevin D. Wells


Assistant Professor

Genetics

Animal Science Research Center
Division Of Animal Sciences
Columbia, MO 65211

  • Phone: (573) 882-1765
  • Email
  • Curriculum Vitae

Education


  • North Carolina State University, Raleigh

    Doctor of Philosophy - Genetics, Minor: Biotechnology
    Department of Genetics and Department of Animal Science, conferred May, 1996.
  • University of Tennessee, Knoxville

    Bachelor of Science - Major: Animal Science; Minor: Biochemistry
    Department of Animal Science, conferred May, 1989.

Publications


Manuscripts

Petters, R.M., Alexander, C.A., Wells, K.D., Collins, E.B., Sommer, J.R., Blanton, M.R., Rojas, G., Hao, Y., Flowers, W.L., Banin, E., Cideciyan, A.C., Jacobson, S.G. and Wong, F. (1997). Genetically engineered large animal model for studying cone photoreceptor survival and degeneration in retinitis pigmentosa. Nature Biotechnology. Oct 1; 15(10): 965-970.

Wells, K.O., Foster, J.A., Moore, K., Pursel, V.G., and Wall, RJ. (1999) Codon Optimization, Genetic Insulation, and an rtTA RepOlier Improve Performance of the Tetracycline Switch. Transgenic Research Oct;8(5):371-81

Du, W., Huang, Z., Flahelty, 1.E., Wells, K., and Payne, G.A. (1999) Green Fluorescent Protein as a reporter to monitor gene expression and food colonization by Aspergillus jlavus. Applied and Environmental Microbiology. Feb; 65(2): 834-836.

Talbot, N.C., Caperna, TJ., Edwards, J.L., Garrett, Wesley, M., Wells, K.D., and Ealy, A.D. (2000) Bovine blastocyst-derived trophectoderm and endoderm cell cultures: interferon tau and transferrin expression as respective in vitro markers Biology O/Reproduction Feb;62(2):235-47.

Wells, K.O. and Powell, A.M. (2000) Blastomeres from somatic cell nuclear transfer embryos are not allocated randomly in chimeric blastocysts Cloning 2(1):9-22.

Kerr, D., Plaut, K., Barmley, 1., Williamson, C., Lax, A., Wells, K.D., Moore, K., Wall, RJ. (2001) Lystostaphin expression in milk confers protection against staphylococcal infection of mammary glands in transgenic mice Nature Biotechnology Jan;19(1):66-70.

Yang J, Ratovitski T, Brady JP, Solomon MB, Wells KO, Wall RJ. (2001) Expression of myostatin pro domain results in muscular transgenic mice. Molecular Reproduction and Development Nov;60(3):351-61.

Dai Y, Vaught TD, Boone J, Chen SH, Phelps CJ, Ball S, Monahan JA, Jobst PM, McCreath KJ, Lamborn AE, Cowell-Lucero JL, Wells KD, Colman A, Polejaeva lA, Ayares DL. (2002) Targeted disruption of the alpha1,3-galactosyltransferase gene in cloned pigs. Nature Biotechnology Mar;20(3):251-5.

Talbot NC, Caperna TJ, Wells KD .. (2002) The PICM-19 Cell Line as an in vitro Model of Liver Bile Ductules: Effects of cAMP Inducers, Biopeptides and pH.
Cells Tissues Organs. 171(2-3):99-116.

Sonstegard T, Capuco AV, White J, Van Tassell CP, Connor EE, Cho J, Sultana R, Shade L, Wray IE, Wells KO, Quackenbush J.(2002) Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index. Mammalian Genome. Jul;13(7):373-9.

Phelps CJ, Koike C, Vaught TO, Boone J, Wells KD, Chen SH, Ball S, Specht SM, Polejaeva lA, Monahan JA, Jobst PM, Shamia SB, Lamborn AE, Garst AS, Moore M, Demetris AJ, Rudert WA, Bottino R, Bertera S, Trucco M, Starzl TE, Dai Y, Ayares DL.(2003) Production of alpha I ,3-galactosyltransferase-deficient pigs. Science. Jan 17;299(5605):411-4.

Mitra A, Foster-Frey J, Rexroad CE 3rd, Wells KD, Wall RJ. (2003) Molecular characterization of lysozyme type II gene in rainbow trout (Oncorhynchus mykiss): evidence of gene duplication. Animal Biotechnology. May;14(1):7-12.

Powell AM, Talbot NC, Wells KD, Kerr DE, Pursel VO, Wall RJ. (2004) Cell donor influences success of producing cattle by somatic cell nuclear transfer. Biology 0/ Reproduction Jul;71 (l ):21 0-6.

Butler JE, Wertz N, Sun J, Wang H, Chardon P, Piumi F, Wells K. (2004) Antibody repertoire development in fetal and neonatal pigs. VII. Characterization of the preimmune kappa light chain repertoire. Journal 0/ Immunology. Dec 1; 173(11 ):6794-805.

Wall RJ, Powell AM, Paape MJ, Kerr DE, Bannerman DD, Pursell VG, Wells KD, Talbot N, Hawk HW (2005) Genetically enhanced cows resist intramammary Staphylococcus aureus infection. Nature Biotechnology, Apr;23( 4):445-51.

Reviews

Petters, R.M. and Wells, K.D. 1993. Culture of Pig Embryos. Journal o/Reproduction and Fertility Supplement 48,61-73.

Wall, RJ., Hyman, P., Kerr, D., Pintado, B., Wells, K. 1997. Transgenic animal technology. Journal 0/ Andrology. May;18(3):236-239.

Wells, K.D. and Wall, RJ. 1999. One gene is not enough: Transgene Detection, Expression, and Control. pp.37-56. In Transgenic Animals in Agriculture, Ed. Murray, J.D., Anderson, G.B., Oberbauer, A.M., and McG10ughlin, M.M. CABI Publishing, New York, NY.

Wells, K.D. 2000. Genome modification for meat science: techniques and applications. American Meat Science Association Conftrence Reciprocal Proceedings July, 2000

Invited commentary

Wells, K, Moore K, Wall R. 1999. Transgene vectors go retro. Nature Biotechnology. Jan;17(1):25-6.

Wells, K., Toward knockout sheep. 2001 Nature Biotechnologyl9, 529 - 530 .

Invited Talks

Scientific Audience

Authors: Wells, Kevin D.
Manuscript Title: Transgenic Livestock: How And Why
Society/Location: Georgetown University
Published/Presented: November, 2000

Authors: Wells, Kevin D.
Manuscript Title: Genome Modification For Meat Science: Techniques And Applications
Society/Location: American Meat Science Association Conference Reciprocal Proceedings Published/Presented: July, 2000

Authors: Wells, Kevin D.
Manuscript Title: Genetically Enhanced Material In Meat Processing
Society/Location: Canadian Meat Council
Published/Presented: Feb,2001

Authors: Kerr, D.E., K.D. Wells, and RJ. Wall.
Manuscript Title: Transgenic Approaches to Prevent Bovine Mastitis.
Society/Location: Annual Meeting of The American Dairy Science Association. Published/Presented: July, 2001.

Authors: Wells, K.D., Williams, W., and Wall, RJ.
Manuscript Title: Regulated Control of Transgene Expression
Society/Location: Transgenic Animal Research Conference Iii Published/Presented: Sept, 2001

Authors: Wells, Kevin D. Manuscript Title: Towards Xenotransplantation: Disruption of Alpha 1,3-Galactosyl Transferase.
Society/Location: Gnotobiotics Society; Raleigh, NC Published/Presented: July, 2002

Authors: Wells, Kevin D.
Manuscript Title: Engineering Pigs to Produce Human Antibodies
Society/Location: 4th Annual Antibody World Summit, Jersey City, NJ Published Presented: July, 2005

Non-Scientist Audience

Authors: Wells, Kevin D.
Title: TRANSGENIC ANIMALS
Where: PA Biotechnology Symposium (Pennsylvania State Fair) When: January,2000

Authors: Wells, Kevin D.
Title: TRANSGENIC ANIMALS AND THE DAIRY INDUSTRY
Where: First Joint Meeting of the Holstein and Jersey Breed associations When: February, 2000

Authors: Wells Kevin D.
Title: HUMAN EMBRYONIC STEM CELLS: RELIGION AND SCIENCE
Where: St. Paul Methodist Church, Christainsburg, V A
When: December, 2004

Published Sequence (26,840 separate Genbank entriesfi'om three species)

Authors: Butler,lE., Wertz,N., Sun,J., Wang,H. and Wells,K.
Title: Characterization of the kappa light chain repertoire.
Comments Repesents 89 entries (AY518081-AY518170)

Authors: Sonstegard,T., Capuco,A.V., White,I., Van Tassell,C.P., Connor,E.E., Cho,J., Sultana,R., Shade,L., Wray,lE., Wells,K.D. and Quackenbush,I.
Title: Analysis of bovine mammary gland EST and functional annotation of the Bos taurus gene index.
Comments: Represents 1,829 entries

Authors: Sonstegard,T.S., Capuco,A.V., Van Tassell,C.P., Ashwell,M.S., Wells,K.D.
Title: Mapping of Expressed Sequence Tags from a normalized bovine mammary gland cDNA library.
Comments: Represents 24,847 entries

Authors: Sonstegard,T.S., Capuco,A.V., Van Tassell,C.P., Ashwell,M.S., Wells,K.D.
Title: Mapping of Expressed Sequence Tags from a normalized bovine mammary gland cDNA library.
Comments: Represents 71 entries (BM878931-BM879001)

Authors: Mitra, A., Wells, K.D., Rexroad, C.E. III and Wall, R.I.
Title: Oncorhynchus mykiss lysozyme II precursor, gene, complete cds.
Comments: Represents two entries.(AF452171 and AF322 I 06)

Authors: Mitra, A., Wells, K.D., Rexroad, C.E. III and Wall, R.I. Title: Oncorhynchus mykiss lysozyme variant mRNA, complete cds.
Comments: AF321519

Authors: Petters,R.M., Alexander,C.A., Wells,K.D., Collins,E.B., Sommer,J.R., Blanton,M.R., Rojas,G.,Hao,Y., Flowers,W.L., Banin,E., Cideciyan,A.V., Jacobson,S.G. and Wong,F Title: Sus scrofa rhodopsin (PIGRHOl) mRNA, complete cds.
Comments: AF008947

Authors: Petters,R.M., Alexander,C.A., Wells,K.D., Collins,E.B., Sommer,J.R., Blanton,M.R., Rojas,G.,Hao,Y.;Flowers,W.L., Banin,E., Cideciyan,A.V., Jacobson,S.G. and Wong,F Title: Genetically engineered large animal model for studying cone photoreceptor survival and degeneration in retinitis pigmentosa
Comments: ~214221

Meeting Abstracts

Wells, K.D., MJ. Tabbaa, and R.M. Petters. 1992. Supplementation of murine embryo culture media with taurine or sorbitol. Biology of Reproduction 46(Supplement 1)135.

Wells, K.D., Z.A. Turner, and R.M. Petters. 1993. The use of ionophore A23187 to manipulate mono/polyspermic fertilization of in vitro matured porcine oocytes. Biology of Reproduction 48(Supplement 1): 170.

Wells, K.D. and R.M. Petters. 1994. Construction of a vector for the genetic manipulation of digestion. Journalof Animal Science 72(Supplement. 2):6.

Wells, K.D., Wall, RJ. and Pursel, V.G. 1996. Modification of the tetracycline system for control of gene expression in transgenic mammals. Beltsville Agricultural Research Center Symposium.

Wells, K.D., Wall, RJ. and Pursel, V.G. Use of green fluorescent protein and a matrix attachment region in the tetracycline system for control of trans gene expression in transgenic mammals. (Meeting abstract, 1997 August 25; Transgenic Animals in Agriculture, Tahoe City, Ca).

Wall, R.I. and Wells, K.D. One gene is not enough. (Meeting presentation, 1997 August 25; Transgenic Animals in Agriculture, Tahoe City, Ca).

Edwards, J.L., Long, C.R., Wells, K., Powell, A. and Rexroad Jr., C.A. 1998. Effects of hyaluronic acid during culture of in vitro derived bovine and porcine embryos. Theriogenology 49(1): 198. (International Embryo Transfer Society)

Wells, K., Powell, A.M., Edwards, lL. and Rexroad Jr., C.E. 1998. Integration of foreign DNA into bovine fetal fibroblasts: factors effecting electroporation efficiency. Theriogenology, 49(1): 395. (International Embryo Transfer Society)

Pursel, V.G., Mitchell, A.D., Bee, G., Wells, K.D., Elsasser, T., McMurtry, J.P., Wall, R.J., Coleman, M.E. and Schwartz, RJ. 1998. Enhanced carcass composition in IGF-I transgenic pigs. (Genetically Engineering and Cloning Animals: science, society, and industry, Park City, UT)

Wells, K.D., Moore, K., Wall, R.I. and Pursel, V.G. 1998. Improvements to the tetracycline transgene control system by introduction of a matrix attachment region, a second reporter gene, and codon optimization. (Genetically Engineering and Cloning Animals: science, society, and industry, Park City, UT)

Pursel, V.G., Wells, K.D., Mitchell, A.D., Elsasser, T., Wall, R.I., Solomon, M.B., Coleman, M.E. and Schwartz, RJ. 1998. Expression ofIGF-I in Skeletal Muscle of Transgenic Swine. (American Society of Animal Science proceedings July 28-31, Denver, Colorado) Journal of Animal Science. 76(Supplement 1):130.

Wells, K., Moore, K., Wall, RJ., and Pursel, V.G. 1999. Expression of the tetracycline transgene control system can be predicted with a second reporter gene and increased by codon optimization or a matrix attachment region. Beltsville Agricultural Research Center Symposium.

Moore, K., Foster, l.A., Wells, K., Wall, RJ. 1999. Isolation and cloning of the murine alpha casein gene for use in gene targeting. Beltsville Agricultural Research Center Symposium.

Maruyama, K., Pursel, V.G., Wells, K.D., Mitchell, A.D. 1999. IGF-I expression levels in striated and cardiac muscle ofIGF-I transgenic swine. (American Society of Animal Science proceedings, Indianapolis, Indiana) Journal of Animal Science. 77(Supplement I): 160. Approved by Area/Institute office 3/5/99.

Pursel, V.G., Mitchell, A.D., Wells, K.D., Wall, RJ., 1999. Coleman, M.E., Schwartz, RJ. Enhanced carcass composition in IGF-I transgenic barrows and gilts. (American Society of Animal Science proceedings, Indianapolis, Indiana) Journal of Animal Science. 77(Supplement 1):157.

Wells, K.D., Powell, A.M., Talbot, N.C., Graninger, P.G., Foster, J.A., Wall, RJ., Pursel, V.G. 1999. Construction of divaricated chimeras with normal trophectoderm and an inner cell mass originating from somatic cell nuclear transfer. (Transgenic Animals in Agriculture, Tahoe City, CA).

Pursel, V.G., Mitchell, A.D., Eastridge, 1.S., Wells, K.D., Wall, RJ., Solomon, M.B., Coleman, M.E., Schwartz, RJ. 1999. Carcass composition resulting from cross of an IGF-I transgenic boar with two lines of hybrid swine. (Transgenic Animals in Agriculture, Tahoe City, CA).

Pursel, V.G., Mitchell, A.D., Wells, K.D., Wall, RJ., Coleman, M.E., Schwartz, RJ. 1999. Alteration of carcass composition in swine with an IGF-I transgene. (Plant and Animal Genome Conference Proceeding, San Deigo, California)

Long, C.R., Pryor, J.H., Wells, K.D., Lane, M., Gardner, D., Looney, C.R. 1999 In vitro development and subsequent pregnancy rates of in vitro produced embryos in various culture media .. Theriogenology 53(1): 2~9. (International Embryo Transfer Society)

Kerr, D., Plaut, K., Barmley, 1., Wells, K.D., Moore, K., Wall, RJ. 2000. Transgenic Mice That Secrete Lysostaphin Into Milk Are Resistant To Mastitis Caused By Staphylococcus Aureus. (American Society of Animal Science proceedings, Baltimore, Maryland) Journal of Animal Science. 78(Supplement 1):39.

Kerr, D.E., K. Plaut, AJ. Bramley, K.D.Wells, K. Moore and RJ. Wall. 2000. Transgenic mice that secrete lysostaphin into milk are resistant to mastitis caused by Staphylococcus aureus. 1. Dairy Sci. 83 (Supp1. 1):39.

Williams, W.R., Foster, l.A., Wells, K.D., Wall, RJ. (2000) Exploring toxicity in the tet inducible system. Beltsville Agricultural Research Center Symposium.

Wells, Kevin D. 2000. Simple, efficient stain for differential staining of trophectoderm and inner cell mass cells. (Genetically Engineering and Cloning Animals: science, society, and industry, Park City, UT)

Kerr, D.E., Bramley, 1., Wells, K.D., Wall, R 2000. Production of transgenic mice resistant to mastitis. Beltsville Agricultural Research Center Symposium.

Sonstegard, T.S., Capuco, A.V., Wells, K.D., Ashwell, M.K., Van Tassel, C.P. 2000. Characterization of gene expression patterns in the bovine mammary gland. Minneapolis, Minnesota.

Sonstegard, T.S., Capuco, A.V., Van Tassel, C.P., Wells, K.D., Ashwell, M.K 2000. Characterization of a normalized cDNA library constructed from bovine mammary tissues. Animal Genetics International Conference Proceedings, San Diego, California.

Powell, A.M., Graninger, P.G., Talbot, N.C., Wells, K.D. 2000. Effects of fibroblast source and tissue-culture medium on success of bovine nuclear transfer with transfected cells. Theriogenology 55(1): 287. (International Embryo Transfer Society)

Kerr, D.E., K.D. Wells and RJ. Wall. 2001. Transgenic approaches to prevent bovine mastitis. J. Dairy Sci. 84 (Suppl. 1):56.

Hruska, KS, Wells, K.D., and Wall, RJ. 2002. Optimization of the tetracycline-responsive system for conditional transgenic livestock. Beltsville Agricultural Research Center Symposium.